Question 1
Bio 200. Practical Lab Exam. EXTRA CREDIT: Steps taken during handling of microorganisms to minimize contamination of the samples, contamination of self and the environment is collectively called ________.
- aseptic technique
Question 2
The following picture is showing the process of gel electrophoresis after PCR is done to a sample. In this gel electrophoresis picture, ________ fragments are being separated by size.
- RNA
- Amino acids
- DNA
- Lipids
Question 3
These are the biochemical test results of an organism whose identity is yet unknown. You suspect it to be one among organisms A, B, C, D, E, F, G or H (see chart below). Based on the 2 biochemical test results below, which organism is it? Make use of the chart provided to conclude. Bio 200. Practical Lab Exam
- G
- F
- D
- E
Question 4
Identify the morphology of the bacteria shown in the Gram stain image.
- Coccus
- Strepto
- Staphylo
- Bacillus Bio 200. Practical Lab Exam
Question 5
Which of the following is true for organism F? Check all that apply.
- it ferments sucrose
- it ferments glucose
- it produces gas
- it produces H2S
- it ferments lactose
Question 6
Based on these results, which antibiotics are useless in treating an E. coli infection? Select ALL that apply.
- A
- B
- C
- D
- E
- F
Question 7
Which of the following tubes show base (alkaline) production?
- A
- B
- D
- C
Question 8
Which of the following tubes show acid production and gas production?
- B
- D
- A
- C
Question 9
What enzyme is being tested for in panel B & C? Bio 200. Practical Lab Exam
- Oxidase
- Catalase
- Dismutase
- Nuclease
- Peroxidase
Question 10
Look at the test result for organisms A, B, C, D and E after incubation at 30C for 48h. Which organisms are negative for indole production? Select ALL that apply.
- D
- B
- A
- E
- C
Question 11
Look at the test result for organism K after incubation at 30C for 48h. What enzyme is produced that causes this organism to turn the agar blue? Bio 200. Practical Lab Exam
- Gelatinase
- Citrase
- Oxidase
- Amylase
Question 12
Look at the test result for organism K after incubation at 30C for 48h. What is the pH indicator used in this medium?
- Phenol Red
- Neutral Red
- Methyl Red Bio 200. Practical Lab Exam
- Bromothymol Blue
Question 13
Which of the following must be done to a sample before any staining procedure can be done to it?
- Fermentation
- Smear preparation
- Sub-culturing
- Respiration
Question 14
This image shows a coagulase test using rabbit plasma. What is the result for Organism A?
- Positive
- Negative
Question 15
Look at the test result for organism K after incubation at 30C for 48h. Based on the test result, has the organism K grown in the tube?
- Yes
- No
