Bio 200 Practical Lab Exam

Question 1

Bio 200. Practical Lab Exam. EXTRA CREDIT: Steps taken during handling of microorganisms to minimize contamination of the samples, contamination of self and the environment is collectively called ________.

• aseptic technique

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Question 2

The following picture is showing the process of gel electrophoresis after PCR is done to a sample. In this gel electrophoresis picture, ________ fragments are being separated by size.

• RNA
• Amino acids
• DNA
• Lipids

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Question 3

These are the biochemical test results of an organism whose identity is yet unknown. You suspect it to be one among organisms A, B, C, D, E, F, G or H (see chart below). Based on the 2 biochemical test results below, which organism is it? Make use of the chart provided to conclude. Bio 200. Practical Lab Exam

• G
• F
• D
• E

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Question 4

Identify the morphology of the bacteria shown in the Gram stain image.

• Coccus
• Strepto
• Staphylo
• Bacillus Bio 200. Practical Lab Exam

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Question 5

Which of the following is true for organism F? Check all that apply.

• it ferments sucrose
• it ferments glucose
• it produces gas
• it produces H2​S
• it ferments lactose

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Question 6

Based on these results, which antibiotics are useless in treating an E. coli infection? Select ALL that apply.

• A
• B
• C
• D
• E
• F

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Question 7

Which of the following tubes show base (alkaline) production?

• A
• B
• D
• C

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Question 8

Which of the following tubes show acid production and gas production?

• B
• D
• A
• C

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Question 9

What enzyme is being tested for in panel B & C? Bio 200. Practical Lab Exam

• Oxidase
• Catalase
• Dismutase
• Nuclease
• Peroxidase

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Question 10

Look at the test result for organisms A, B, C, D and E after incubation at 30C for 48h. Which organisms are negative for indole production? Select ALL that apply.

• D
• B
• A
• E
• C

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Question 11

Look at the test result for organism K after incubation at 30C for 48h. What enzyme is produced that causes this organism to turn the agar blue? Bio 200. Practical Lab Exam

• Gelatinase
• Citrase
• Oxidase
• Amylase

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Question 12

Look at the test result for organism K after incubation at 30C for 48h. What is the pH indicator used in this medium?

• Phenol Red
• Neutral Red
• Methyl Red Bio 200. Practical Lab Exam
• Bromothymol Blue

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Question 13

Which of the following must be done to a sample before any staining procedure can be done to it?

• Fermentation
• Smear preparation
• Sub-culturing
• Respiration

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Question 14

This image shows a coagulase test using rabbit plasma. What is the result for Organism A?

• Positive
• Negative

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Question 15

Look at the test result for organism K after incubation at 30C for 48h. Based on the test result, has the organism K grown in the tube?

• Yes
• No